Comparison of a Radioimmunoassay with an Enzymatic Assay for Gentamicin
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چکیده
منابع مشابه
Latex agglutination inhibition card test for gentamicin assay: clinical evaluation and comparison with radioimmunoassay and bioassay.
Gentamicin levels were determined in 100 serum specimens by a new latex agglutination inhibition card test, a radioimmunoassay (RIA), and a bioassay. Correlation coefficients determined by linear regression analysis demonstrated that the levels obtained by the latex agglutination inhibition card test had a high degree of correlation with the RIA and could be performed much faster and more econo...
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An enzymatic, radiochemical assay procedure for measuring serum gentamicin by use of gentamicin 3-acetyl-transferase and [acetyl-14C]acetyl-Coenzyme A is described and evaluated. The enzyme stoichiometrically and quantitatively transfers a radioactive label to the analyte during a 10-min incubation at 37 degrees C. The labeled gentamicin is then absorbed onto phosphocellulose paper discs, which...
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A method is described for the assay of gentamicin using the enzyme gentamicin adenylyltransferase derived from an R-factor-carrying strain of Escherichia coli. The reaction involves adenylylation of the gentamicin with ((14)C)-ATP to form a radioactively labelled product. The technique is compared with the plate diffusion and the urease methods. The adenylylation technique gives results compara...
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15 صفحه اولDouble-osmotic-shock procedure to prepare gentamicin adenine mononucleotide transferase for the enzymatic assay of gentamicin.
The release of gentamicin adenine mononucleotide transferase during single, cold, osmotic-shock treatment of Escherichia coli K-12 W677/HJR66 is not always maximal. The quantity of gentamicin adenine mononucleotide transferase in osmotic extracts could be significantly increased when a double-shock procedure was performed.
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ژورنال
عنوان ژورنال: Antimicrobial Agents and Chemotherapy
سال: 1975
ISSN: 0066-4804,1098-6596
DOI: 10.1128/aac.7.1.107